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trim33 knockout esc lines  (Addgene inc)


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    Addgene inc trim33 knockout esc lines
    a) Volcano plot showing the enrichment of proteins immunoprecipitated from DOX-treated over untreated ESC DUXBL-LG by using two independent ESC lines from a representative DUXBL IP-MS experiment. Enriched proteins in DOX-treated cells are highlighted in red. b) Western blot analysis of the indicated proteins performed with FLAG immunoprecipitates obtained from untreated or DOX-treated ESC DUXBL-LG . c) Immunofluorescence analysis of endogenous DUXBL and TRIM24 in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two WT and TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. d) Immunofluorescence analysis of endogenous <t>TRIM33</t> and TRIM24 in untreated or DOX-treated LTR-RFP reporter ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two ESC DUX clones were performed but one representative experiment is shown. e) High-throughput imaging quantification of the number of DUXBL foci per cell (upper panel), the total intensity of DUXBL foci per cell (middle panel) and the total area of DUXBL foci per cell (lower panel) in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . Center lines indicate mean values. n=2000; Percentages above the threshold (dotted line) are indicated. Relevant p values are shown from one-tailed unpaired t -tests. Three independent experiments using at least two WT or TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. f) Immunofluorescence analysis of endogenous DUXBL and TRIM24 at different timepoints in untreated or DOX-treated WT ESC DUX . Time since the addition of DOX is indicated. DAPI was used to visualize nuclei. Scale bar, 20 μm.
    Trim33 Knockout Esc Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trim33+knockout+esc+lines/TRIM33+(TRIM33B-c100)+(Plasmid+%2398243)/bio_rxiv__2022__09__19__508541-219-5-28
    Average 93 stars, based on 6 article reviews
    trim33 knockout esc lines - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "The homeobox transcription factor DUXBL controls exit from totipotency"

    Article Title: The homeobox transcription factor DUXBL controls exit from totipotency

    Journal: bioRxiv

    doi: 10.1101/2022.09.19.508541

    a) Volcano plot showing the enrichment of proteins immunoprecipitated from DOX-treated over untreated ESC DUXBL-LG by using two independent ESC lines from a representative DUXBL IP-MS experiment. Enriched proteins in DOX-treated cells are highlighted in red. b) Western blot analysis of the indicated proteins performed with FLAG immunoprecipitates obtained from untreated or DOX-treated ESC DUXBL-LG . c) Immunofluorescence analysis of endogenous DUXBL and TRIM24 in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two WT and TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. d) Immunofluorescence analysis of endogenous TRIM33 and TRIM24 in untreated or DOX-treated LTR-RFP reporter ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two ESC DUX clones were performed but one representative experiment is shown. e) High-throughput imaging quantification of the number of DUXBL foci per cell (upper panel), the total intensity of DUXBL foci per cell (middle panel) and the total area of DUXBL foci per cell (lower panel) in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . Center lines indicate mean values. n=2000; Percentages above the threshold (dotted line) are indicated. Relevant p values are shown from one-tailed unpaired t -tests. Three independent experiments using at least two WT or TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. f) Immunofluorescence analysis of endogenous DUXBL and TRIM24 at different timepoints in untreated or DOX-treated WT ESC DUX . Time since the addition of DOX is indicated. DAPI was used to visualize nuclei. Scale bar, 20 μm.
    Figure Legend Snippet: a) Volcano plot showing the enrichment of proteins immunoprecipitated from DOX-treated over untreated ESC DUXBL-LG by using two independent ESC lines from a representative DUXBL IP-MS experiment. Enriched proteins in DOX-treated cells are highlighted in red. b) Western blot analysis of the indicated proteins performed with FLAG immunoprecipitates obtained from untreated or DOX-treated ESC DUXBL-LG . c) Immunofluorescence analysis of endogenous DUXBL and TRIM24 in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two WT and TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. d) Immunofluorescence analysis of endogenous TRIM33 and TRIM24 in untreated or DOX-treated LTR-RFP reporter ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two ESC DUX clones were performed but one representative experiment is shown. e) High-throughput imaging quantification of the number of DUXBL foci per cell (upper panel), the total intensity of DUXBL foci per cell (middle panel) and the total area of DUXBL foci per cell (lower panel) in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . Center lines indicate mean values. n=2000; Percentages above the threshold (dotted line) are indicated. Relevant p values are shown from one-tailed unpaired t -tests. Three independent experiments using at least two WT or TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. f) Immunofluorescence analysis of endogenous DUXBL and TRIM24 at different timepoints in untreated or DOX-treated WT ESC DUX . Time since the addition of DOX is indicated. DAPI was used to visualize nuclei. Scale bar, 20 μm.

    Techniques Used: Immunoprecipitation, Protein-Protein interactions, Western Blot, Immunofluorescence, Clone Assay, High Throughput Screening Assay, Imaging, One-tailed Test

    Related Articles

    Knock-Out:

    Article Title: The homeobox transcription factor DUXBL controls exit from totipotency
    Article Snippet: .. To generate DUXBL, TRIM24 and TRIM33 knockout ESC lines, we independently infected a suspension of ESC with lentiviral supernatants, generated by using LentiCRISPRv2 (gift from Feng Zhang, 52961, Addgene), encoding sgRNAs designed to target the corresponding protein (Supplementary Table 8 for sgRNA sequences). .. To generate DOX-inducible DUXBL LG and DUXBL SM ESC lines as well as ESC DUX-FKBP lines or ESC DUX-2XHA lines, the corresponding sequences were PCR amplified from cDNA including a 3XFLAG for DUXBL LG or an HA-tag for DUXBL SM and subcloned by Gibson assembly into the plasmid PB-TRE-dCas9-VPR (Gift from George Church, 63800, Addgene), after removing the dCas9-VPR insert.

    Infection:

    Article Title: The homeobox transcription factor DUXBL controls exit from totipotency
    Article Snippet: .. To generate DUXBL, TRIM24 and TRIM33 knockout ESC lines, we independently infected a suspension of ESC with lentiviral supernatants, generated by using LentiCRISPRv2 (gift from Feng Zhang, 52961, Addgene), encoding sgRNAs designed to target the corresponding protein (Supplementary Table 8 for sgRNA sequences). .. To generate DOX-inducible DUXBL LG and DUXBL SM ESC lines as well as ESC DUX-FKBP lines or ESC DUX-2XHA lines, the corresponding sequences were PCR amplified from cDNA including a 3XFLAG for DUXBL LG or an HA-tag for DUXBL SM and subcloned by Gibson assembly into the plasmid PB-TRE-dCas9-VPR (Gift from George Church, 63800, Addgene), after removing the dCas9-VPR insert.

    Suspension:

    Article Title: The homeobox transcription factor DUXBL controls exit from totipotency
    Article Snippet: .. To generate DUXBL, TRIM24 and TRIM33 knockout ESC lines, we independently infected a suspension of ESC with lentiviral supernatants, generated by using LentiCRISPRv2 (gift from Feng Zhang, 52961, Addgene), encoding sgRNAs designed to target the corresponding protein (Supplementary Table 8 for sgRNA sequences). .. To generate DOX-inducible DUXBL LG and DUXBL SM ESC lines as well as ESC DUX-FKBP lines or ESC DUX-2XHA lines, the corresponding sequences were PCR amplified from cDNA including a 3XFLAG for DUXBL LG or an HA-tag for DUXBL SM and subcloned by Gibson assembly into the plasmid PB-TRE-dCas9-VPR (Gift from George Church, 63800, Addgene), after removing the dCas9-VPR insert.

    Generated:

    Article Title: The homeobox transcription factor DUXBL controls exit from totipotency
    Article Snippet: .. To generate DUXBL, TRIM24 and TRIM33 knockout ESC lines, we independently infected a suspension of ESC with lentiviral supernatants, generated by using LentiCRISPRv2 (gift from Feng Zhang, 52961, Addgene), encoding sgRNAs designed to target the corresponding protein (Supplementary Table 8 for sgRNA sequences). .. To generate DOX-inducible DUXBL LG and DUXBL SM ESC lines as well as ESC DUX-FKBP lines or ESC DUX-2XHA lines, the corresponding sequences were PCR amplified from cDNA including a 3XFLAG for DUXBL LG or an HA-tag for DUXBL SM and subcloned by Gibson assembly into the plasmid PB-TRE-dCas9-VPR (Gift from George Church, 63800, Addgene), after removing the dCas9-VPR insert.



    Similar Products

    trim33 knockout esc lines  (Addgene inc)
    93
    Addgene inc trim33 knockout esc lines
    a) Volcano plot showing the enrichment of proteins immunoprecipitated from DOX-treated over untreated ESC DUXBL-LG by using two independent ESC lines from a representative DUXBL IP-MS experiment. Enriched proteins in DOX-treated cells are highlighted in red. b) Western blot analysis of the indicated proteins performed with FLAG immunoprecipitates obtained from untreated or DOX-treated ESC DUXBL-LG . c) Immunofluorescence analysis of endogenous DUXBL and TRIM24 in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two WT and TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. d) Immunofluorescence analysis of endogenous <t>TRIM33</t> and TRIM24 in untreated or DOX-treated LTR-RFP reporter ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two ESC DUX clones were performed but one representative experiment is shown. e) High-throughput imaging quantification of the number of DUXBL foci per cell (upper panel), the total intensity of DUXBL foci per cell (middle panel) and the total area of DUXBL foci per cell (lower panel) in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . Center lines indicate mean values. n=2000; Percentages above the threshold (dotted line) are indicated. Relevant p values are shown from one-tailed unpaired t -tests. Three independent experiments using at least two WT or TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. f) Immunofluorescence analysis of endogenous DUXBL and TRIM24 at different timepoints in untreated or DOX-treated WT ESC DUX . Time since the addition of DOX is indicated. DAPI was used to visualize nuclei. Scale bar, 20 μm.
    Trim33 Knockout Esc Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trim33+knockout+esc+lines/TRIM33+(TRIM33B-c100)+(Plasmid+%2398243)/bio_rxiv__2022__09__19__508541-219-5-28
    Average 93 stars, based on 1 article reviews
    trim33 knockout esc lines - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a) Volcano plot showing the enrichment of proteins immunoprecipitated from DOX-treated over untreated ESC DUXBL-LG by using two independent ESC lines from a representative DUXBL IP-MS experiment. Enriched proteins in DOX-treated cells are highlighted in red. b) Western blot analysis of the indicated proteins performed with FLAG immunoprecipitates obtained from untreated or DOX-treated ESC DUXBL-LG . c) Immunofluorescence analysis of endogenous DUXBL and TRIM24 in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two WT and TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. d) Immunofluorescence analysis of endogenous TRIM33 and TRIM24 in untreated or DOX-treated LTR-RFP reporter ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two ESC DUX clones were performed but one representative experiment is shown. e) High-throughput imaging quantification of the number of DUXBL foci per cell (upper panel), the total intensity of DUXBL foci per cell (middle panel) and the total area of DUXBL foci per cell (lower panel) in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . Center lines indicate mean values. n=2000; Percentages above the threshold (dotted line) are indicated. Relevant p values are shown from one-tailed unpaired t -tests. Three independent experiments using at least two WT or TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. f) Immunofluorescence analysis of endogenous DUXBL and TRIM24 at different timepoints in untreated or DOX-treated WT ESC DUX . Time since the addition of DOX is indicated. DAPI was used to visualize nuclei. Scale bar, 20 μm.

    Journal: bioRxiv

    Article Title: The homeobox transcription factor DUXBL controls exit from totipotency

    doi: 10.1101/2022.09.19.508541

    Figure Lengend Snippet: a) Volcano plot showing the enrichment of proteins immunoprecipitated from DOX-treated over untreated ESC DUXBL-LG by using two independent ESC lines from a representative DUXBL IP-MS experiment. Enriched proteins in DOX-treated cells are highlighted in red. b) Western blot analysis of the indicated proteins performed with FLAG immunoprecipitates obtained from untreated or DOX-treated ESC DUXBL-LG . c) Immunofluorescence analysis of endogenous DUXBL and TRIM24 in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two WT and TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. d) Immunofluorescence analysis of endogenous TRIM33 and TRIM24 in untreated or DOX-treated LTR-RFP reporter ESC DUX . DAPI was used to visualize nuclei. Scale bars, 20 μm. Dashed regions are zoomed-in in the insets. Three independent experiments using two ESC DUX clones were performed but one representative experiment is shown. e) High-throughput imaging quantification of the number of DUXBL foci per cell (upper panel), the total intensity of DUXBL foci per cell (middle panel) and the total area of DUXBL foci per cell (lower panel) in untreated or DOX-treated LTR-RFP reporter WT or TRIM24 KO ESC DUX . Center lines indicate mean values. n=2000; Percentages above the threshold (dotted line) are indicated. Relevant p values are shown from one-tailed unpaired t -tests. Three independent experiments using at least two WT or TRIM24 KO ESC DUX clones were performed but one representative experiment is shown. f) Immunofluorescence analysis of endogenous DUXBL and TRIM24 at different timepoints in untreated or DOX-treated WT ESC DUX . Time since the addition of DOX is indicated. DAPI was used to visualize nuclei. Scale bar, 20 μm.

    Article Snippet: To generate DUXBL, TRIM24 and TRIM33 knockout ESC lines, we independently infected a suspension of ESC with lentiviral supernatants, generated by using LentiCRISPRv2 (gift from Feng Zhang, 52961, Addgene), encoding sgRNAs designed to target the corresponding protein (Supplementary Table 8 for sgRNA sequences).

    Techniques: Immunoprecipitation, Protein-Protein interactions, Western Blot, Immunofluorescence, Clone Assay, High Throughput Screening Assay, Imaging, One-tailed Test